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Quartz Halogen Lamp: A 100-watt high intensity lamp operating at 3000ºK provides excitation over a wide spectrum, refer to Figure 6 for distribution of radiatted energy. This lamp is recommended for use when excitation wavelengths are equal to or greater then 450 nm.
Optical Filters: Both the promary and secondary filter holders can accommodate a combination of filters up to 10 mm in thickness. Over fifty different glass color filters covering a broad spectral range are availiable. See figures 8 and 9 (Filter information). Combinations of these filters and interference types will satisfy any requirement. It is recommened that the samples' excitation and emission spectra be obtained to aid in the selection of optical filters.
Photomultiplier Tubes: The S4 responce Photomultiplier Tube (PMT) is provided with each flourometer unless otherwise specified. This tube's spectral responce, together with one having extended red sensitivity are shown in Figure 7. In applications where higher responces are required for different spectral areas, other PMT tubes may be selected.
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Mercury
Lamp: A 100-watt, high intensity, mercury vapor lamp provides excitation energy.
Substantial spectral continuum is available from 220 to 650nm, with much higher
energy lines at discreet wavelengths. Figure 5 indicates the approximate
distribution of the radiated energy from 200 to 650 nm. Major mercury lines are:
254, 265, 313, 366, 405, 436, 546 and 577nm.